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Cloning, mapping and gene product identification of rhaT from Escherichia coli K12

✍ Scribed by Baldoma, L


Book ID
122186300
Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
204 KB
Volume
72
Category
Article
ISSN
0378-1097

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The lkyB gene of Escherichia coli K12 has been cloned from the Clarke and Carbon colony bank by selecting a ColEa plasmid conferring cholic acid resistance to lkyB mutants. The lkyB gene was localized on hybrid plasmid pJC778 by analysis of mutated plasmids generated by Tn5 insertions. Restriction a