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Cloning, Heterologous Expression, and Functional Characterization of a Chitinase Gene,Lbchi32, fromLimonium bicolor

✍ Scribed by Zhi Hua Liu; Chuan Ping Yang; Xiao Tian Qi; Li Li Xiu; Yu Cheng Wang


Publisher
Springer
Year
2010
Tongue
English
Weight
565 KB
Volume
48
Category
Article
ISSN
0006-2928

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Cloning and expression of a chitinase ge
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Recombinant clones from a cDNA library of an Aphanocladium album chitinase-overproducing mutant strain were isolated by screening with antiserum against a 39 kDa chitinase purified from this hyperparasitic fungus. Analysis of the isolated positive clones indicated that most of them carried the same