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Cloning, Expression and Purification ofMicrocystis viridisLectin inEscherichia coli

โœ Scribed by Yuqin Li; Xiaoxia Liao; Gu Chen; Yeeleng Yap; Xuewu Zhang

Book ID
107581857
Publisher
Humana Press Inc
Year
2010
Tongue
English
Weight
412 KB
Volume
47
Category
Article
ISSN
1073-6085

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A 1.5 kb plasmid-encoded lysostaphin gene fragment of Staphylococcus staphylolyticus was amplified by polymerase chain reaction OPCR) and cloned in Escherichia coli by using plasmid pET29b(+) as an expression vector. By optimizing culture conditions, the activities of lysostaphin were expressed as 6