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Cloning, Expression, and Purification of Histidine-Tagged preS Domains of Hepatitis B Virus

✍ Scribed by Elena Núñez; Xin Wei; Carmen Delgado; Ignacio Rodrı́guez-Crespo; Belén Yélamos; Julián Gómez-Gutiérrez; Darrell L. Peterson; Francisco Gavilanes

Book ID
115645785
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
149 KB
Volume
21
Category
Article
ISSN
1046-5928

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The complete (encoding 119 aminon acids, aa) or partial (encoding the N-terminal 90 aa) preS1 region gene of hepatitis B virus (HBV) was fused to the 3'-end of glutathione-S-transferase (GST) gene and expressed at 37 °C under the control of the inducible tac promoter in E. coli. The results showed t