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Cloning, expression and characterization ofmeso-2,3-butanediol dehydrogenase fromKlebsiella pneumoniae

✍ Scribed by Gen Lin Zhang, Cui Wei Wang, Chun Li


Book ID
118796068
Publisher
Springer Netherlands
Year
2012
Tongue
English
Weight
249 KB
Volume
34
Category
Article
ISSN
0141-5492

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A NAD-dependent (R)-2,3-butanediol dehydrogenase (EC 1.1.1.4), selectively catalyzing the oxidation at the (R)-center of 2,3-butanediol irrespective of the absolute configuration of the other carbinol center, was isolated from cell extracts of the yeast Saccharomyces cerevisiae. Purification was ach