Sequence analysis of the gene for and characterization of d-acetoin forming meso-2,3-butanediol dehydrogenase of Klebsiella pneumoniae expressed in Escherichia coli

Optimization of expression of dhaT gene

Optimization of expression of dhaT gene encoding 1,3-propanediol oxidoreductase from Klebsiella pneumoniae in Escherichia coli using the methods of uniform design and regression analysis

✍ Yang Cao; Qirong Xia; Baishan Fang 📂 Article 📅 2005 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 89 KB

## Abstract This paper is concerned with the optimization of expression conditions of 1,3‐propanediol oxidoreductase (PDOR) using the uniform design (UD) method and regression analysis. The concentration of the inducer isopropyl‐β‐D‐thiogalactopyranoside (IPTG), the induction temperature and the in