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Cloning, Expression and Characterization of a Thiolase Gene fromClostridium pasteurianum

✍ Scribed by Yonghong Meng; Jilun Li

Publisher
Springer Netherlands
Year
2006
Tongue
English
Weight
312 KB
Volume
28
Category
Article
ISSN
0141-5492

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Molecular cloning of a new endoglucanase
Molecular cloning of a new endoglucanase gene fromClostridium cellulolyticumand its expression inEscherichia coli
✍ Seigo Shima; Yasuo Igarashi; Tohru Kodama πŸ“‚ Article πŸ“… 1991 πŸ› Springer 🌐 English βš– 481 KB

Two genes coding for endoglucanase activity in Clostridium cellulolyticum were cloned and expressed in Escherichia coli by using plasmid pUC18. The sizes of two fragments harbouring endoglucanase genes are 4.4 kb and 2.0 kb, respectively. The 2.0-kb fragment was identical with a reported D N A fragm