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Cloning and targeted disruption of two polygalacturonase genes in Penicillium olsonii

✍ Scribed by Florian Wagner; Heike Kusserow; Wilhelm Schäfer

Book ID
117516365
Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
327 KB
Volume
186
Category
Article
ISSN
0378-1097

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✍ Šiekštel, Rimantas; Bartkevičitūtė, Danguolė; Sasnauskas, Kęstutis 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 502 KB

The yeast Kluyveromyces marxianus strain BKM Y-719 produces an efficient pectin-degrading endopolygalacturonase (EPG) that cleaves the internal -1,4--glycosidic linkages to yield oligomers of varying sizes. The EPG1 gene encoding this industrially important EPG was cloned by using the polymerase ch