𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Cloning and sequencing of theClostridium perfringensenterotoxin gene

✍ Scribed by Marijke Damme-Jongsten; Karel Wernars; Servé Notermans

Publisher
Springer Netherlands
Year
1989
Tongue
English
Weight
415 KB
Volume
56
Category
Article
ISSN
0003-6072

No coin nor oath required. For personal study only.

✦ Synopsis

Several gene banks of Clostridium perfringens in E. coli were constructed. Using a mixture of synthetic 29-mer DNA probes clones were selected containing inserts from the C. perfringens gene coding for the enterotoxin. This has allowed sequencing of the complete gene and its flanking regions. The decuded amino acid sequence (320 a.a.) was found to differ at several sites from the sequence published previously by others. Two 40-mer DNA-probes were used to detect the toxin gene in C. perfringens strains isolated from the faeces of different non-symptomatic animals. Only 6% of the strains were found to possess the gene.

📜 SIMILAR VOLUMES

Cloning and expression of theClostridium
Cloning and expression of theClostridium thermosulfurogenesD-xylose isomerase gene (xyLA) inSaccharomyces cerevisiae
✍ Catharine J. Moes; Isak S. Pretorius; Willem H. Zyl 📂 Article 📅 1996 🏛 Springer Netherlands 🌐 English ⚖ 503 KB

A recombinant strain of Saccharomyces cerevisiae containing the bacterial Dxylose isomerase (xylA) gene of Clostridium thermosulfirogenes was constructed. The .+A gene has high thermal stability, functions in an anaerobic environment and has a G+C content of 38.9%, which should render it a good cand