Plasmid DNA containing the replication origin of the Escherichia coli chromosome (oriC) has been shown to be inefficient as a template for DNA synthesis in vitro when isolated from dam mutants. Here, we extend this study to hemimethylated oriC plasmids and to replication in dam-3 mutant enzyme extra
Cloning and sequencing of the replication origin (oriC) of theSpiroplasma citrichromosome and construction of autonomously replicating artificial plasmids
✍ Scribed by Fengchun Ye; Joël Renaudin; Joseph-Marie Bové; Frédéric Laigret
- Publisher
- Springer
- Year
- 1994
- Tongue
- English
- Weight
- 749 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0343-8651
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Anacystis nidulans contains two cryptic plasmids of 8.0 (pANS) and 48.5 (pANL) kilobasepairs (kbp). A clone bank of the large plasmid pANL consisting of 7 Barn HI fragments has been established. The cloned fragments were used as radioactive probes to Barn HI, Sal L Hind III and Eco R1 digests of pAN
We describe the molecular cloning of BglII fragments of the hybrid plasmid pRS5 (pSC101 and EcoRI fragments of F; f7, f5, f3 and f6). The clones isolated were examined for the expression of F-specified replication, incompatibility, mobilization and inhibition of T7 bacteriophage multiplication. Prot
Heat shock proteins have been shown to be involved in many cellular processes in procaryotic and eucaryotic cells. Using an in vitro DNA replication assay, we show that DNA synthesis initiated at the chromosomal origin of replication of Escherichia coli (oriC) is considerably reduced in enzyme extra