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Cloning and Sequencing of the Gene of Site-Specific Nickase N.BspD6I

✍ Scribed by T. A. Perevyazova; E. A. Rogulin; L. A. Zheleznaya; N. I. Matvienko


Book ID
111555021
Publisher
Springer
Year
2003
Tongue
English
Weight
79 KB
Volume
68
Category
Article
ISSN
0006-2979

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Several gene banks of Clostridium perfringens in E. coli were constructed. Using a mixture of synthetic 29-mer DNA probes clones were selected containing inserts from the C. perfringens gene coding for the enterotoxin. This has allowed sequencing of the complete gene and its flanking regions. The de