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Cloning and sequencing of potato virus Y (Hungarian isolate) genomic RNA

✍ Scribed by Thole, Vera; Dalmay, Tamás; Burgyán, József; Balázs, Ervin


Book ID
122723998
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
964 KB
Volume
123
Category
Article
ISSN
0378-1119

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Cloning and mapping of the potato virus
✍ Miri Karchi; Ilan Sela; Orit Edelbaum; Assaf Ori; Benjamin Raccah; Arie Rosner; 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 784 KB

Full-length cDNA of genomic RNA of potato virus Y (PVY) was cloned in one piece into a lambda vector. The order of the EcoRI and SalI fragments of the inserted cDNA was determined. This is the first report of the cloning of a long, expressible, potyvirus genome. The availability of such a clone is a