Cloning and mapping of the potato virus Y genome and its in vitro expression

✍ Scribed by Miri Karchi; Ilan Sela; Orit Edelbaum; Assaf Ori; Benjamin Raccah; Arie Rosner; Yehuda Stram

• Publisher: Springer
• Year: 1990
• Tongue: English
• Weight: 784 KB
• Volume: 4
• Category: Article
• ISSN: 0920-8569
• DOI: 10.1007/bf00265631

✦ Synopsis

Full-length cDNA of genomic RNA of potato virus Y (PVY) was cloned in one piece into a lambda vector. The order of the EcoRI and SalI fragments of the inserted cDNA was determined. This is the first report of the cloning of a long, expressible, potyvirus genome. The availability of such a clone is a prerequisite for any further study of the molecular biology of this group of viruses, as they are expressed into a self-processed primary polyprotein.