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Cloning and sequence of the Rhizobium leguminosarum biovar phaseoli fixA gene

✍ Scribed by Jan Michiels; Jos Vanderleyden

Book ID
115720030
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
190 KB
Volume
1144
Category
Article
ISSN
0005-2728

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Molecular cloning and nucleotide sequenc
Molecular cloning and nucleotide sequence of the Rhizobium phaseoli recA gene
✍ Michiels, Jan ;Vande Broek, Ann ;Vanderleyden, Jos πŸ“‚ Article πŸ“… 1991 πŸ› Springer 🌐 English βš– 522 KB

A recombinant lambda phage carrying the recA gene of Rhizobium phaseoli was isolated from a R. phaseoli genomic library by complementation of the Fec- phenotype of the recombinant phage in Escherichia coli. When expressed in E. coli, the cloned recA gene was shown to restore resistance to both UV ir