Cloning and complete nucleotide sequence
β
Nohno, Tsutomu ;Saito, Taiichi ;Hong, Jen-shiang
π
Article
π
1986
π
Springer
π
English
β 1011 KB
The glutamine permease operon encoding the high-affinity transport system of glutamine in Escherichia coli could be cloned in one of the mini F plasmids, but not in pBR322 or pACYC184, by selection for restoration of the Gln+ phenotype, the ability to utilize glutamine as a sole carbon source. We de