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Cloning, expression and immunogenicity of the translation initiation factor 3 homologue of Brucella abortus

✍ Scribed by Marcela González; Edilia Andrews; Hugo Folch; Darwin Sáez; Alex Cabrera; Paulina Salgado; Angel Oñate


Book ID
116569325
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
607 KB
Volume
214
Category
Article
ISSN
0171-2985

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## Abstract A gene coding for a protein homologous to a translation initiation factor of eukaryotes, eIF5A, was cloned from __Methanococcus jannaschii__, a hyperthermophile with an optimum growth temperature of 85 °C. The protein was overexpressed, purified and crystallized. The crystals were obtai