✦ LIBER ✦

Cloning and mapping of theCYS4gene ofSaccharomyces cerevisiae

✍ Scribed by Bun-ichiro Ono; Chinatsu Heike; Yukie Yano; Toyomi Inoue; Kazuhide Naito; Satoru Nakagami; Akio Yamane

Publisher: Springer-Verlag
Year: 1992
Tongue: English
Weight: 489 KB
Volume: 21
Category: Article
ISSN: 0172-8083
DOI: 10.1007/bf00351684

No coin nor oath required. For personal study only.

✦ Synopsis

A D N A fragment containing the C YS4 gene of Saccharomyces cerevisiae was isolated from a genomic library. The cloned fragment hybridized to the transverse-alternating-field-electrophoresis band corresponding to chromosomes VII and XV. According to the 2 ~tm D N A chromosome-loss procedure, the cys2 and cys4 mutations, which are linked together and co-operatively confer cysteine dependence, were assigned to chromosome VIL By further mapping involving tetrad analysis, the cys2-cys4 pair was localized between SUP77 (SUP166) and ado3 on the right arm of chromosome VII.

📜 SIMILAR VOLUMES

Cloning and biochemical characterization

Cloning and biochemical characterization ofLYS5gene ofSaccharomyces cerevisiae

✍ Christopher W. Borell; J. K. Bhattacharjee 📂 Article 📅 1988 🏛 Springer-Verlag 🌐 English ⚖ 587 KB

In Saccharomyces cerevisiae, the functions of two unlinked genes (LYS2 and LYS5) are required for the synthesis of the lysine biosynthetic enzyme, a-aminoadipate reductase. The LYS5 gene of S. cerevisiae was cloned by functional complementation of a lys5 mutant, X4004-3A, using a YEp24 plasmid libra

Cloning and integrative deletion of theR

Cloning and integrative deletion of theRAD6gene ofSaccharomyces cerevisiae

✍ Martin Kupiec; Giora Simchen 📂 Article 📅 1984 🏛 Springer-Verlag 🌐 English ⚖ 702 KB

Three overlapping plasmids were isolated from a YEp24 library, which restore Rad + functions to rad6-1 and tad6-3 mutants. Different subclones were made and shown to integrate by homologous recombination at the RAD6 site on chromosome VII, thus verifying the cloned DNA segments to be the RAD6 gene a

Cloning and Expression of Two Chitin Dea

Cloning and Expression of Two Chitin Deacetylase Genes ofSaccharomyces cerevisiae

✍ MISHRA, CHITRA; SEMINO, CARLOS E.; McCREATH, KENNETH J.; DE LA VEGA, HUMBERTO; J 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 381 KB

Chitin deacetylase (EC 3.5.1.41), which hydrolyses the N-acetamido groups of N-acetyl--glucosamine residues in chitin, has been demonstrated in crude extracts from sporulating Saccharomyces cerevisiae. Two S. cerevisiae open reading frames (ORFs), identified by the Yeast Genome Project, have protei

Cloning and physical characterization of

Cloning and physical characterization of linked lysine genes (LYS4, LYS15) ofSaccharomyces cerevisiae

✍ Li Wang; Susumu Okamoto; J. K. Bhattacharjee 📂 Article 📅 1989 🏛 Springer-Verlag 🌐 English ⚖ 528 KB

The glycerol kinase (GUT1) gene ofSaccha

The glycerol kinase (GUT1) gene ofSaccharomyces cerevisiae: cloning and characterization

✍ P. Pavlik; M. Simon; T. Schuster; H. Ruis 📂 Article 📅 1993 🏛 Springer-Verlag 🌐 English ⚖ 516 KB

Cloning and mapping of the RAD50 gene of

Cloning and mapping of the RAD50 gene of Saccharomyces cerevisiae

✍ Kupiec, Martin ;Simchen, Giora 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 703 KB

The RAD50 gene was cloned as a 4.8 kb fragment in the 2 mu derived plasmid pFL1. The gene resides in a 3.9 kb segment that was subcloned into the plasmid YRp7. The cloned gene complements the deficiency caused by the rad50-1 mutation with respect to gamma-rays, MMS resistance and UV-induced mitotic