𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Cloning and integrative deletion of theRAD6gene ofSaccharomyces cerevisiae

✍ Scribed by Martin Kupiec; Giora Simchen

Publisher
Springer-Verlag
Year
1984
Tongue
English
Weight
702 KB
Volume
8
Category
Article
ISSN
0172-8083

No coin nor oath required. For personal study only.

✦ Synopsis

Three overlapping plasmids were isolated from a YEp24 library, which restore Rad + functions to rad6-1 and tad6-3 mutants. Different subclones were made and shown to integrate by homologous recombination at the RAD6 site on chromosome VII, thus verifying the cloned DNA segments to be the RAD6 gene and not a suppressor. The gene resides in a 1.15 kb fragment, which restores Rad + levels of resistance to U.V., MMS and 7rays to both rad6-1 and rad6-3 strains. It also restores sporulation ability to rad6-1 diploids.

Integrative deletion of the RAD6 gene was shown not to be completely lethal to the yeast. Our results suggest that the RAD6 gene has some cell cycle-specific function(s), probably during late S phase.

πŸ“œ SIMILAR VOLUMES

Cloning and mapping of theCYS4gene ofSac
Cloning and mapping of theCYS4gene ofSaccharomyces cerevisiae
✍ Bun-ichiro Ono; Chinatsu Heike; Yukie Yano; Toyomi Inoue; Kazuhide Naito; Satoru πŸ“‚ Article πŸ“… 1992 πŸ› Springer-Verlag 🌐 English βš– 489 KB

A D N A fragment containing the C YS4 gene of Saccharomyces cerevisiae was isolated from a genomic library. The cloned fragment hybridized to the transverse-alternating-field-electrophoresis band corresponding to chromosomes VII and XV. According to the 2 ~tm D N A chromosome-loss procedure, the cys

Cloning and Expression of Two Chitin Dea
Cloning and Expression of Two Chitin Deacetylase Genes ofSaccharomyces cerevisiae
✍ MISHRA, CHITRA; SEMINO, CARLOS E.; McCREATH, KENNETH J.; DE LA VEGA, HUMBERTO; J πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 381 KB

Chitin deacetylase (EC 3.5.1.41), which hydrolyses the N-acetamido groups of N-acetyl--glucosamine residues in chitin, has been demonstrated in crude extracts from sporulating Saccharomyces cerevisiae. Two S. cerevisiae open reading frames (ORFs), identified by the Yeast Genome Project, have protei

Cloning and biochemical characterization
Cloning and biochemical characterization ofLYS5gene ofSaccharomyces cerevisiae
✍ Christopher W. Borell; J. K. Bhattacharjee πŸ“‚ Article πŸ“… 1988 πŸ› Springer-Verlag 🌐 English βš– 587 KB

In Saccharomyces cerevisiae, the functions of two unlinked genes (LYS2 and LYS5) are required for the synthesis of the lysine biosynthetic enzyme, a-aminoadipate reductase. The LYS5 gene of S. cerevisiae was cloned by functional complementation of a lys5 mutant, X4004-3A, using a YEp24 plasmid libra

Cloning and expression on a multicopy ve
Cloning and expression on a multicopy vector of five invertase genes ofSaccharomyces cerevisiae
✍ Stefan Hohmann; Friedrich K. Zimmermann πŸ“‚ Article πŸ“… 1986 πŸ› Springer-Verlag 🌐 English βš– 837 KB

Six unlinked loci for invertase structural genes are known in the yeast Saccharornyces cerevisiae: SUC1-SUC5 and SUC7. These genes are similar in structure and expression but not identical. Different yeast strains possess none, one or several of these genes. We have isolated the genes SUC1-SUC5, su