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Cloning and heterologous expression of a bacteriocin sakacin P fromLactobacillus sakeiinEscherichia coli

✍ Scribed by Haiqin Chen; Fengwei Tian; Shuo Li; Yan Xie; Hao Zhang; Wei Chen


Book ID
113025461
Publisher
Springer
Year
2012
Tongue
English
Weight
329 KB
Volume
94
Category
Article
ISSN
1432-0614

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The glucoamylase gene from lactobacillus amylovorus was cloned and expressed in Escherichia coli. A genomic DNA library from Laclobacillus amylovorus was prepared by partially digesting genomic DNA with EC&I and ligating random fragments to the EcoRl digested cloning vector, pZErO-I .I. Three E. co/