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Cloning and functional characterization of CYP71D14, a root-specific cytochrome P450 fromPetunia hybrida

✍ Scribed by Hiromasa Imaishi; Tomomi Matushita

Book ID
107480197
Publisher
Springer-Verlag
Year
2008
Tongue
English
Weight
108 KB
Volume
30
Category
Article
ISSN
0137-5881

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The cloning of small GTP-binding proteins from Petunia hybrida was performed using a PCR-based strategy. Degenerate primers were designed from the DTAGQE and FMETSA consensus sequences. Three different cDNAs were amplified. The deduced polypeptide sequences PhPCRGP1 and PhPCRGP2 were homologous to R