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Cloning and expression of the secA gene of a marine bacterium, Vibrio alginolyticus, and analysis of its function in Escherichia coli

โœ Scribed by Ei-ichi Kunioka; Shin-ichi Matsuyama; Hajime Tokuda


Book ID
117523247
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
523 KB
Volume
216
Category
Article
ISSN
0378-1119

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Cloning and expression in Escherichia co
โœ Paul, Kalidas ;Ghosh, Subrata K. ;Das, Jyotirmoy ๐Ÿ“‚ Article ๐Ÿ“… 1986 ๐Ÿ› Springer ๐ŸŒ English โš– 645 KB

A library containing more than 80% of the Vibrio cholerae genome was constructed by cloning BamH1 restriction fragments into pBR322. Using interspecific complementation of an Escherichia coli recA mutant with plasmids containing the gene bank of V. cholerae, a recA-like gene was identified. The reco