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Cloning, functional expression and primary characterization of Vibrio parahaemolyticus K+/H+ antiporter genes in Escherichia coli

✍ Scribed by Martha V. Radchenko; Rungaroon Waditee; Sawako Oshimi; Masahiro Fukuhara; Teruhiro Takabe; Tatsunosuke Nakamura


Book ID
110832782
Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
412 KB
Volume
59
Category
Article
ISSN
0950-382X

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A library containing more than 80% of the Vibrio cholerae genome was constructed by cloning BamH1 restriction fragments into pBR322. Using interspecific complementation of an Escherichia coli recA mutant with plasmids containing the gene bank of V. cholerae, a recA-like gene was identified. The reco