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Cloning and expression of the gene of the human chymotrypsin-like kallikrein-7 protease inEscherichia coliand isolation of the recombinant protein

โœ Scribed by A. I. Zakabunin; O. V. Mishukova; E. A. Khrapov; D. S. Sergievichev; U. A. Boyarskikh; E. D. Sverdlov; M. L. Filipenko


Book ID
111498207
Publisher
Allerton Press, Inc.
Year
2007
Tongue
English
Weight
271 KB
Volume
22
Category
Article
ISSN
0891-4168

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โœ Won Hee Jang; Eun Kyung Kim; Hwanghee Blaise Lee; Jae Hoon Chung; Ook Joon Yoo ๐Ÿ“‚ Article ๐Ÿ“… 1996 ๐Ÿ› Springer Netherlands ๐ŸŒ English โš– 877 KB

A gene, aprP, encoding an extracellular alkaline serine protease from a newly isolated Pseudomonas sp. KFCC 10818 was cloned and characterized. Nucleotide sequence analysis revealed an open reading frame of 1,266 nucleotides which could encode a polypeptide comprised of 422 amino acids. The C-termin