The E. coli polA (+) gene has been subcloned from a specialised Ξ» transducing phage onto a low copy number plasmid. Plasmid-encoded DNA polymerase I was synthesised at 2 to 3 times the wild-type E. coli level, and was biochemically indistinguishable from chromosomally-encoded protein. It was able to
β¦ LIBER β¦
Cloning and expression of the flagellar hook gene on hybrid plasmids in minicells
β Scribed by SIMON, M.; MATSUMURA, P.; SILVERMAN, M.
- Book ID
- 109703967
- Publisher
- Nature Publishing Group
- Year
- 1977
- Tongue
- English
- Weight
- 426 KB
- Volume
- 265
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/265758a0
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