The E. coli polA (+) gene has been subcloned from a specialised Ξ» transducing phage onto a low copy number plasmid. Plasmid-encoded DNA polymerase I was synthesised at 2 to 3 times the wild-type E. coli level, and was biochemically indistinguishable from chromosomally-encoded protein. It was able to
β¦ LIBER β¦
Cloning and expression of the essential gene for poly(A) polymerase from S. cerevisiae
β Scribed by Lingner, Joachim; Kellermann, Josef; Keller, Walter
- Book ID
- 109779141
- Publisher
- Nature Publishing Group
- Year
- 1991
- Tongue
- English
- Weight
- 481 KB
- Volume
- 354
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/354496a0
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