Limitations to the amplification and sta
β
C. H. Fann; F. Guirgis; G. Chen; M. S. Lao; J. M. Piret
π
Article
π
2000
π
John Wiley and Sons
π
English
β 222 KB
π 1 views
Chinese hamster ovary cell production of recombinant tissue-type plasminogen activator (t-PA) was increased by amplification of cotransfected dihydrofolate reductase cDNA using stepwise adaptation to increasing methotrexate (MTX) concentrations. The highest producing clones were isolated at 5 Β΅M MTX