𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Cloning and expression of human tissue-type plasminogen activator cDNA in chinese hamster ovary cells

✍ Scribed by Peitang Huang; Xiuying Xu; Jianhui Guo; Jun Cheng


Book ID
119038198
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
81 KB
Volume
63
Category
Article
ISSN
0049-3848

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


Limitations to the amplification and sta
✍ C. H. Fann; F. Guirgis; G. Chen; M. S. Lao; J. M. Piret πŸ“‚ Article πŸ“… 2000 πŸ› John Wiley and Sons 🌐 English βš– 222 KB πŸ‘ 1 views

Chinese hamster ovary cell production of recombinant tissue-type plasminogen activator (t-PA) was increased by amplification of cotransfected dihydrofolate reductase cDNA using stepwise adaptation to increasing methotrexate (MTX) concentrations. The highest producing clones were isolated at 5 Β΅M MTX