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Cloning and expression of bacteriophage FMV lysocyme gene in cells of yeastsSaccharomyces cerevisiaeandPichia pastoris

โœ Scribed by D. G. Kozlov; S. E. Cheperegin; A. V. Chestkov; V. N. Krylov; Yu. D. Tsygankov


Book ID
110199914
Publisher
SP MAIK Nauka/Interperiodica
Year
2010
Tongue
English
Weight
382 KB
Volume
46
Category
Article
ISSN
1022-7954

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## Abstract An ovalbumin gene was cloned from Chinese quail (__Coturnix coturnix__) oviduct by RTโ€PCR and then inserted into the __P. pastoris__ genome under the control of the methanol inducible 5โ€ฒ alcohol oxidase (AOX) promoter. The recombinant __P. pastoris__ strain was demonstrated to be able t