Cloning and expression of a Melanocarpus albomyces steryl esterase gene in Pichia pastoris and Trichoderma reesei

Cloning of a novel ovalbumin gene from q

Cloning of a novel ovalbumin gene from quail oviduct and its heterologous expression in Pichia pastoris

✍ Shaohui Yang; Gong Chen; Xinchun Yu; Minggang Li; Jiehua Wang 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 283 KB 👁 1 views

## Abstract An ovalbumin gene was cloned from Chinese quail (__Coturnix coturnix__) oviduct by RT‐PCR and then inserted into the __P. pastoris__ genome under the control of the methanol inducible 5′ alcohol oxidase (AOX) promoter. The recombinant __P. pastoris__ strain was demonstrated to be able t