Cloning and expression of alpha-amylase from Bacillus amyloliquefaciens in a stable plasmid vector in Lactobacillus plantarum
โ Scribed by S. Jones; P. J. Warner
- Book ID
- 115271612
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 374 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0266-8254
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A 6.4 Kb HindIII fragment of Bacillus stearothermophilus DY-5 DNA cloned in Escherichia coli using pBR322 as a vector was shown to direct the synthesis of a thermophilic alpha-amylase. In attempts to reduce the size of the insert, the alpha-amylase gene was shown to be contained in a 3.1 Kb HindIII