A set of dual promoter vectors for high throughput cloning, screening, and protein expression in eukaryotic and prokaryotic systems from a single plasmid

Construction of a series of vectors for

Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins fromMycobacterium tuberculosis

✍ Huajun Qin; Jian Hu; Yuanzhi Hua; Shridhar V Challa; Timothy A Cross; Fei P Gao 📂 Article 📅 2008 🏛 BioMed Central 🌐 English ⚖ 899 KB

## Background One of the major challenges for membrane protein structural genomics is establishing high-throughput cloning and expression screening methods to obtain enough purified protein in a homogeneous preparation for structural and functional studies. Here a series of ligation independent clo

[Methods in Molecular Biology] High Thro

[Methods in Molecular Biology] High Throughput Protein Expression and Purification Volume 498 || A Family of LIC Vectors for High-Throughput Cloning and Purification of Proteins

✍ Doyle, Sharon A. 📂 Article 📅 2009 🏛 Humana Press ⚖ 299 KB

PCR-linked in vitro expression: a novel

PCR-linked in vitro expression: a novel system for high-throughput construction and screening of protein libraries

✍ Suang Rungpragayphan; Hideo Nakano; Tsuneo Yamane 📂 Article 📅 2003 🏛 Elsevier Science 🌐 English ⚖ 212 KB

4745062 Plasmid vectors for cloning and

4745062 Plasmid vectors for cloning and expression of a protein in a microorganism, comprising at least one promoter for expression of beta-glucosidase in yeasts; microorganisms containing these plasmids; a fermentation process and the enzymes obtained

📂 Article 📅 1988 🏛 Elsevier Science 🌐 English ⚖ 66 KB

4745062 Plasmid vectors for cloning and

4745062 Plasmid vectors for cloning and expression of a protein in a microorganism, comprising at least one promoter for expression of beta-glucosidase in yeasts; microorganisms containing these plasmids; a fermentation process and the enzymes obtained

📂 Article 📅 1988 🏛 Elsevier Science 🌐 English ⚖ 65 KB