Cloning and nucleotide sequencing of a l
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B. W. Thanomsub; C. Boonchird; V. Meevootisom
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Article
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1996
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Springer
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English
β 510 KB
A lipase gene from B. subtilis WRRL-B558 was cloned in Escherichia coli JM109 using pBluescript as a vector plasmid. Two methods were combined to screen for the lipase-producing clone. The first was done by overlaying the screening plates with Ξ²-naphthylacetate and Fast Blue BB dye. Positive clones