✦ LIBER ✦

Cloning and expression in Escherichia coli K-12 of the biosynthetic dehydroquinase function of the arom cluster gene from the eucaryote, Aspergillus nidulans

✍ Scribed by Kinghorn, James R. ;Hawkins, Alastair R.

Publisher: Springer
Year: 1982
Tongue: English
Weight: 817 KB
Volume: 186
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00422927

No coin nor oath required. For personal study only.

✦ Synopsis

A 1.35 Md DNA HindIII fragment containing part of the arom gene cluster or cluster gene of Aspergillus nidulans encoding biosynthetic dehydroquinase (5-dehydroquinate hydrolyase) has been cloned in plasmid pBR322 on the basis of functional expression in Escherichia coli. The fungal fragment on pBR322, designated pHK29, complements a corresponding E. coli dehydroquinase structural gene (aroD) mutation. pHK29 contains one BamHI, HpaII, PstI, SmaI, XhoI and surprisingly, one HindIII site since pHK29 hybrid Aspergillus DNA is a HindIII fragment itself. The biosynthetic dehydroquinase activity extracted from E. coli strains, containing pHK29, had properties similar to those of the enzyme activity from Aspergillus. The protein specified by pHK29 appears to be 80 kd. No increase of dehydroquinase activity was found in polynucleotide phosphorylase deficient strains (pnp) of E. coli.

📜 SIMILAR VOLUMES

Cloning and expression of the exbB gene

Cloning and expression of the exbB gene of Escherichia coli K-12

✍ Eick-Helmerich, Katrin ;Hantke, Klaus ;Braun, Volkmar 📂 Article 📅 1987 🏛 Springer 🌐 English ⚖ 842 KB

The exbB locus of Escherichia coli is involved in the uptake of certain iron(III) siderophore compounds, of vitamin B12 and of certain colicins. Outer membrane receptor proteins are essential constituents of the corresponding uptake systems. The DNA carrying the exbB locus was cloned into pACYC184 a

Cloning of the ARO cluster gene of Neuro

Cloning of the ARO cluster gene of Neurospora crassa and its expression in Escherichia coli

✍ Catcheside, D. E. A. ;Storer, P. J. ;Klein, B. 📂 Article 📅 1985 🏛 Springer 🌐 English ⚖ 699 KB

Molecular cloning and expression of the

Molecular cloning and expression of the biodegradative threonine dehydratase gene (tdc) of Escherichia coli K12

✍ Goss, Thomas J. ;Datta, Prasanta 📂 Article 📅 1985 🏛 Springer 🌐 English ⚖ 944 KB

The biodegradative threonine dehydratase gene (tdc) of Escherichia coli was cloned by isolating a dehydratase negative mutant after Tn5 mutagenesis, cloning the tdc::Tn5 DNA into pBR322 and then replacing the Tn5 element on the plasmid in vivo. Subcloning and nucleotide sequence data revealed two di

Cloning of the tyrocidine synthetase 1 g

Cloning of the tyrocidine synthetase 1 gene from Bacillus brevis and its expression in Escherichia coli

✍ Marahiel, Mohamed A. ;Krause, Michael ;Skarpeid, Hans-Jacob 📂 Article 📅 1985 🏛 Springer 🌐 English ⚖ 907 KB

The entire structural gene for tyrocidine synthetase 1 from Bacillus brevis ATCC 8185 has been cloned and expressed in Escherichia coli. Transformed E. coli cells were screened for their ability to produce tyrocidine synthetase 1 by in situ immunoassay using antibodies against gramicidin S synthetas

Cloning of the excC and excD genes invol

Cloning of the excC and excD genes involved in the release of periplasmic proteins by Escherichia coli K12

✍ Lazzaroni, Jean-Claude ;Fognini-Lefebvre, Nicole ;Portalier, Raymond 📂 Article 📅 1989 🏛 Springer 🌐 English ⚖ 507 KB

Control of arg gene expression in salmon

Control of arg gene expression in salmonella typhimurium by the arginine repressor from Escherichia coli K-12

✍ Gardner, Michelle M. ;Hennig, Douglas O. ;Kelln, Rod A. 📂 Article 📅 1983 🏛 Springer 🌐 English ⚖ 540 KB

The regulation of synthesis of arg enzymes in Salmonella typhimurium by the arginine repressor of Escherichia coli K-12 has been reevaluated using a strain of S. typhimurium in which the argR gene was rendered nonfunctional by inserting the translocatable tetracycline-resistance element Tn10 into th