From a pollen tube cDNA library of Petunia inflata, we isolated cDNA clones encoding a protein, PPE1, which exhibits sequence similarity with plant, bacterial, and fungal pectin esterases. Genomic clones containing the PPE1 gene were isolated using cDNA for PPE1 as a probe, and comparison of the cDN
Cloning and characterization of PO22, a pollen-expressed gene in alfalfa
โ Scribed by Yongzhong Wu; Xiao Qui; Sarah Du; Larry Erickson
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 318 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0167-4412
No coin nor oath required. For personal study only.
โฆ Synopsis
Genomic and cDNA clones have been isolated for a new gene (PO22) expressed in mature pollen of alfalfa. PO22 represents a single-or low-copy gene containing 4 introns, 2 transcriptional start sites, and 6oding for a predicted protein of 340 amino acids with no signal peptide and a coiled coil structure of 39 amino acids near the carboxyl end. The amino acid sequence of PO22 is 38% identical to that of an early nodule-specific gene (ENOD8), also from alfalfa.
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