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Characterization of a pollen-expressed gene encoding a putative pectin esterase ofPetunia inflata

✍ Scribed by Jing-Hong Mu; Joseph P. Stains; Teh-hui Kao

Publisher: Springer
Year: 1994
Tongue: English
Weight: 515 KB
Volume: 25
Category: Article
ISSN: 0167-4412
DOI: 10.1007/bf00043881

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✦ Synopsis

From a pollen tube cDNA library of Petunia inflata, we isolated cDNA clones encoding a protein, PPE1, which exhibits sequence similarity with plant, bacterial, and fungal pectin esterases. Genomic clones containing the PPE1 gene were isolated using cDNA for PPE1 as a probe, and comparison of the cDNA and genomic sequences revealed the presence of a single intron in the PPEI gene. During pollen development, PPE1 m R N A was first detected in anthers containing uninucleate microspores; it reached the highest level in mature pollen and persisted at a high level in in vitro germinated pollen tubes. The observed expression pattern of the PPE1 gene suggests that its product may play a role in pollen germination and/or tube growth.

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