Cloning and characterization of gentamicin-resistance genes from Micromonospora purpurea and Micromonospora rosea
✍ Scribed by Kelemen, Gabriella H.; Cundliffe, Eric; Financsek, István
- Book ID
- 120937121
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 827 KB
- Volume
- 98
- Category
- Article
- ISSN
- 0378-1119
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When DNA fragments from Micromonospora purpurea (the producer of gentamicin) were cloned in Streptomyces lividans, a gentamicin-resistant strain was obtained in which the ribosomes were highly resistant both to gentamicin and to kanamycin. Reconstitution analysis revealed that such resistance result
Resistance to aminoglycoside antibiotics in Micromonospora purpurea (the producer of gentamicin C complex), Streptomyces tenebrarius (the nebramycin producer) and Streptomyces tenjimariensis (which makes istamycin) occurs at the level of the ribosome. Reconstitution analysis has revealed, in each ca