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Cloning and characterization of an α1-antitrypsin like gene 12 kb downstream of the genuine α1-antitrypsin gene

✍ Scribed by M.H. Hofker; M. Nelen; E.C. Klasen; T. Nukiwa; D. Curiel; R.G. Crystal; R.R. Frants


Book ID
117058098
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
600 KB
Volume
155
Category
Article
ISSN
0006-291X

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A method for detection of the M and Z alleles of the alpha l-antitrypsin deficiency (AAT) gene has been developed using denaturing gradient gel electrophoresis (DGGE) of DNA samples amplified in vitro by the polymerase chain reaction. Amplification of the 90 nucleotides surrounding the Z mutation si