The complete human T-cell leukemia virus type I (HTLV-I) env gene was inserted into an expression cassette containing the adenovirus 5 major late promoter (Ad5-MLP). Recombinant Ad5-HTLV-I-env was obtained by homologous recombination in 293 cells simultaneously transfected by the expression cassette
Cloning and analysis of recombinant plasmids containing genes forAspergillus nidulans5 S rRNA
β Scribed by Ewa Bartnik; Katarzyna Strugala; Piotr P. Stepien
- Publisher
- Springer-Verlag
- Year
- 1981
- Tongue
- English
- Weight
- 301 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0172-8083
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β¦ Synopsis
Genes coding for 5S rRNA were found to be dispersed in the Aspergillus nidulans genome. Three different recombinant plasmids hybridizing to 5S rRNA were isolated and their restriction enzyme maps were established.
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The chloroplast 5S rRNA gene of the brown alga Pylaiella littoralis (L.) Kjellm has been cloned and sequenced. The gene is located 23 bp downstream from the 3' end of the 23S rRNA gene. The sequence of the gene is as follows: GGTCTTG GTGTTTAAAGGATAGTGGAACCACATTGAT CCATATCGAACTCAATGGTGAAACATTATT ACAG