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Cloned ß-galactosidase gene ofEscherichia coliis expressed in the yeastSaccharomyces cerevisiae

✍ Scribed by Jean-Jacques Panthier; Philippe Fournier; Henri Heslot; Alain Rambach

Publisher
Springer-Verlag
Year
1980
Tongue
English
Weight
441 KB
Volume
2
Category
Article
ISSN
0172-8083

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✦ Synopsis

The expression of the lacZ gene of E. coli in S. cerevisiae has been studied. The enzymatic activity coded by the lacZ gene in E. coli, β-galactosidase, is detectable in yeast cells harboring a chimeric plasmid carrying the gene. On the basis of size and immunological criteria, no difference was detected between the Coli-in-yeast β-galactosidase and the E. coli enzyme.

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