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Clonal transformation of human leukocytes by Epstein-barr virus in soft agar

✍ Scribed by Naoki Yamamoto; Yorio Hinuma

Publisher
John Wiley and Sons
Year
1976
Tongue
French
Weight
518 KB
Volume
17
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

The B95‐8 strain of Epstein‐Barr virus (EBV) induced colony formation of human umbilical cord‐blood leukocytes in soft agar medium. One‐hit response relationship between the number of colonies and the virus dose was observed with high dilutions of the virus preparation. However, there was a presumed cell‐killing effect with low dilutions of virus. The colonies were similarly induced, but with a lower efficiency, in adult peripheral blood leukocyte cultures infected with the virus. The colony‐forming activity of EBV was neutralized by anti‐EBV‐positive but not by negative human sera. The cells in colonies were capable of growing continuously and carried EBV‐associated nuclear antigen. Thus, it was evident that the colony formation was caused by clonal transformation by EBV.

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