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Clonal nature of murine hemopoietic blast cell colonies

✍ Scribed by Fujimori, Yoshihiro ;Hara, Hiroshi ;Taguchi, Tetsuya ;Kitamura, Yukihiko ;Nagai, Kiyoyasu

Book ID
104720549
Publisher
Springer-Verlag
Year
1988
Weight
848 KB
Volume
57
Category
Article
ISSN
1432-0584

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✦ Synopsis

Murine hemopoietic blast cell colonies obtained from spleen cells of 5-fluorouracil (5-FU)-treated mice give rise to many multilineage colonies including granulocyte - erythrocyte - macrophage - megakaryocyte (GEMM) colonies in secondary cultures. Progenitor cells for blast cell colonies are considered to be more primitive than colony forming units (CFU)-GEMM. To determine whether they are clonal, we examined the phosphoglycerate kinase-1 (PGK-1) isozyme type of colonies originally grown from spleen cells of 5-FU-treated mice which had PGK-1 isozyme mosaicism. PGK assays of whole secondary colonies derived from one blast cell colony showed that they were either of type A or type B but not both. These results suggest that murine hemopoietic blast cell colonies are clonal.

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