Clonal cytogenetic abnormalities in Hodgkin's disease

✍ Scribed by Dr. Marc Ladanyi; Nasser Z. Parsa; Kenneth Offit; Mitchell S. Wachtel; Daniel A. Filippa; Suresh C. Jhanwar

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 667 KB
• Volume: 3
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/gcc.2870030408

✦ Synopsis

Cytogenetic studies of Hodgkin's disease (HD), in contrast t o those of non-Hodgkin's lymphoma (NHL), have been limited t o small numbers of cases with infrequently recurring aberrations, underscoring the need for additional studies in establishing a coherent cytogenetic picture of HD. Over a 6%-year period, we received 95 specimens of HD for cytogenetic analysis.

Analyzable chromosome preparations were obtained in 70 cases, of which 57 (8 I %) showed only normal metaphases. In the remaining I 3 cases (I 9%), karyotypic abnormalities were observed that were nonclonal in 3 and clonal in 10. The latter I0 cases included 6 of the nodular sclerosis subtype, 3 mixed cellularity, and I lymphocyte-depleted; 8 of the specimens were obtained pretreatment and 2 posttreatment. Two of the cases had a clonal numerical aberration, monosomy I 7 in one and trisomy I 3 in the other, as their sole abnormality. The remaining 8 cases showed complex karyotypes with multiple structural rearrangements; in 3 of these, the abnormal clone was near-tetraploid. Bands involved more than once included I p36, I q2 I, and 4q35, each in 2 cases. Arms involved more than once included 6q (6q I 3,6q23), 9p (9p I 3,9p2 I), and 5q (5q I5,5q35). Three patients had loss of part or all of 6q (del(6)(q I 3),de1(6)(q23),i(6p)). Bands I4q32 and I8q2 I were not involved in any case, contrary t o some previous reports. Our results confirm the frequent occurrence of I p. I q, and 6q abnormalities in HD. In addition, we have identified a 5q35 breakpoint, which has recently been shown t o be highly specific for Ki-I -positive NHL in a case of typical nodular sclerosis HD. Its presence in H D may represent a cytogenetic link between the two entities, which are immunophenotypically related but clinically and histologically distinct.