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Cleavage and sequence recognition of 2,6-diaminopurine-containing DNA by site-specific endonucleases

✍ Scribed by Miklós Szekeres; Andrey V. Matveyev

Book ID
115919068
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
518 KB
Volume
222
Category
Article
ISSN
0014-5793

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The chloroplast ribosomal intron of Chlamydomonas reinhardtii encodes a sequence-specific DNA endonuclease (I-CreI), which is most probably involved in the mobility of this intron. Here we show that I-CreI generates a 4 bp staggered cleavage just downstream of the intron insertion site. The I-CreI r