The pyruvate metabolism of a Lactococcus lactis subsp. lactis biovar diacetylactis mutant deficient in :-acetolactate decarboxylase and its wild-type strain was studied during batch cultivations. A chemically defined medium was used containing glucose as carbon-and energy-source. The :-acetolactate
Citrate metabolism in Lactococcus lactis subsp. lactis var. diacetylactis strains
β Scribed by Libudzisz, Z. ;Galewska, E.
- Book ID
- 102217608
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 414 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0027-769X
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β¦ Synopsis
The formation of diacetyl, acetoin, 2,3-hutylene glycol, acetaldehyde, ethanol and lactic acid during 24 h of cultivation in milk with 0.19 and 0.5 % of citrate has been studied. Depending on the strain, bacteria produced 1.5 -1.9 mg of diacetyl, 212 -31 1 mg of acetoin and 137 -156 mg of butylene glycol in 1 I milk. An increase of the citrate concentration in milk to 0.5 % resulted in an increase in the production of diacetyl from 58 to 74 % and of acetoin by 2.8 -3.7 times. The strains of distinct activity of acetoin reductase produced in these conditions 2.3 -2.7 times as much as 2,3-hutylene glycol. The recovery of citrate in the from of C4-compounds ranged from 76 to 98 96, yet barely 0.18 -0.44 % in the from of diacetyl. Increased concentration of citrate in milk stimulated the production of diacetyl and acetaldchyde to the similar extent, thereby it did not result in the deterioration of organoleptic qualities of starters and milk products. Within the doses used citrate did not significantly affect growth and acidifying activity of the bacteria.
π SIMILAR VOLUMES
The effect of starvation on citrate metabolism by fully grown cells of S/wp~o(~occus lactis subsp. diace/>,/ar/is was studied by three different procedures. An 8-hour starvation period in fermented medium decreased citrate uptake rate but diacetyl and acetoin levels remained constant over a fermenta