✦ LIBER ✦

Circular polymerase extension cloning for high-throughput cloning of complex and combinatorial DNA libraries

✍ Scribed by Quan, Jiayuan; Tian, Jingdong

Book ID: 109946841
Publisher: Nature Publishing Group
Year: 2011
Tongue: English
Weight: 574 KB
Volume: 6
Category: Article
ISSN: 1750-2799
DOI: 10.1038/nprot.2010.181

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Circular Polymerase Extension Cloning of

Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways

✍ Quan, Jiayuan; Tian, Jingdong; Ho, Paulo Lee 📂 Article 📅 2009 🏛 Public Library of Science 🌐 English ⚖ 250 KB

Circular rapid amplification of cDNA end

Circular rapid amplification of cDNA ends for high-throughput extension cloning of partial genes

✍ Glenn K Fu; Jonathan T Wang; Junming Yang; Janice Au-Young; Laura L Stuve 📂 Article 📅 2004 🏛 Elsevier Science 🌐 English ⚖ 159 KB

A one pot high throughput cloning method

A one pot high throughput cloning method for construct optimization, creation of libraries of construct variants, and DNA shuffling

✍ S. Marillonnet 📂 Article 📅 2009 🏛 Elsevier 🌐 English ⚖ 77 KB

Large scale purification of linear plasm

Large scale purification of linear plasmid DNA for efficient high throughput cloning

✍ Dr. Marjolaine Noirclerc-Savoye; Benoit Gallet; Florent Bernaudat; Thierry Verne 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 256 KB

Optimization and automation of fluoresce

Optimization and automation of fluorescence-based DNA hybridization for high-throughput clone mapping

✍ Patrik Scholler; Steffen Heber; Dr. Jörg D. Hoheisel 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 595 KB

Large-scale hybridization-based genome mapping projects, such as the production of sequence-ready physical clone maps, call for robust and cheap DNA labeling techniques that are amenable to automation. We routinely use a highthroughput protocol based on fluorescence detection. DNA probes are labeled

From extensive clone libraries to compre

From extensive clone libraries to comprehensive DNA arrays for the efficient and simultaneous detection and identification of orchid mycorrhizal fungi

✍ Bart Lievens; Stefan van Kerckhove; Annelies Justé; Bruno P.A. Cammue; Olivier H 📂 Article 📅 2010 🏛 Elsevier Science 🌐 English ⚖ 608 KB