Chromosome-mediated transfer and amplification of an altered mouse dihydrofolate reductase gene
โ Scribed by Daniel A. Haber; Robert T. Schimke
- Book ID
- 112558760
- Publisher
- Springer
- Year
- 1982
- Tongue
- English
- Weight
- 752 KB
- Volume
- 8
- Category
- Article
- ISSN
- 1572-9931
No coin nor oath required. For personal study only.
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Recombinant Chinese hamster ovary (CHO) cells expressing a high-level of chimeric antibody against S surface antigen of hepatitis B virus were obtained by co-transfection of heavy and light chain cDNA expression vectors into dihydrofolate reductase (dhfr)-deficient CHO cells and subsequent gene ampl
Previously, the highest producing (HP) recombinant CHO subclones isolated at various methotrexate (MTX) levels showed different antibody production stability during long-term culture, although they were clonally derived from CS13 transformant. In this study, genetic basis for their difference in ant