Chromosome alterations in 21 non-small cell lung carcinomas

✍ Scribed by Ikuo Miura; Jill M. Siegfried; James Resau; Steven M. Keller; Jian-Yuan Zhou; Dr. Joseph R. Testa

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 886 KB
• Volume: 2
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/gcc.2870020411

✦ Synopsis

1. R.)

Cytogenetic analysis was performed on I 6 primary tumors, 2 effusions, and 3 cell lines from 2 I patients with non-small cell lung cancer (NSCLC). In 20 patients specimens were obtained prior t o initiating cytotoxic therapy. Extensive clonal chromosome alterations were found in all cases. The most frequent numerical changes were polysomy 7 and polysomy 20 (each seen in I2 specimens). In addition, tumor cells from another six cases exhibited partial trisomy 7, with the shortest region of overlap (SRO) at 7p I I-p I 3. Rearrangements of chromosomes I , 3,6,8, I I, 15, 17, and I9 were each observed in nine or more tumors.

Breakpoints were clustered at several chromosomal sites, including I p I 3, 3p I 3, I5p I I-q I I, I7p I I, and 19q I 3. Recurrent loss involving I p. 3p, 6q, I I p, I5p, I7p, and 19q were each seen in at least eight cases. The SRO of 3p losses was at band 3~21. Double minute chromosomes were found in three tumors. Overall, our findings indicate that even though karyotypes in newly diagnosed NSCLC are very complex, recurrent cytogenetic changes can be identified. The high incidence of loss of I7p (I 4 of 2 I specimens) appears t o be compatible with reports implicating the TP53 gene (at band I7p I 3) as a frequent site for genetic alteration in lung cancer. Moreover, the recurrence of loss of 3p (I 2 cases) and I I p ( I0 cases) is also consistent with recent molecular evidence. The existence of other "hot spots" for cytogenetic change, particularly those involving specific regions on chromosomes 7, 15, and 19, warrants further molecular investigation of these sites in NSCLC.