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Chromosomal rearrangements during human epidermal keratinocyte differentiation

✍ Scribed by Narasimharao V. Marella; Brandon Seifert; Priyadharsini Nagarajan; Satrajit Sinha; Ronald Berezney

Publisher: John Wiley and Sons
Year: 2009
Tongue: English
Weight: 621 KB
Volume: 221
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.21855

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Undifferentiated human epidermal keratinocytes are self‐renewing stem cells that can be induced to undergo a program of differentiation by varying the calcium chloride concentration in the culture media. We utilize this model of cell differentiation and a 3D chromosome painting technique to document significant changes in the radial arrangement, morphology, and interchromosomal associations between the gene poor chromosome 18 and the gene rich chromosome 19 territories at discrete stages during keratinocyte differentiation. We suggest that changes observed in chromosomal territorial organization provides an architectural basis for genomic function during cell differentiation and provide further support for a chromosome territory code that contributes to gene expression at the global level. J. Cell. Physiol. J. Cell. Physiol. 221: 139–146, 2009. © 2009 Wiley‐Liss, Inc.

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