Proteins were isolated from purified yeast chromatin and subjected to two-dimensional electrophoresis. The cellular and the chromosomal content of the major nonhistone proteins was measured. Two polypeptides of molecular weights 55,000 and 53,000, identified as α and β tubulin, and a polypeptide of
Chromosomal proteins inSaccharomyces cerevisiae
✍ Scribed by Kay Gulløv; Jørgen Friis
- Book ID
- 104777258
- Publisher
- Springer-Verlag
- Year
- 1980
- Tongue
- English
- Weight
- 281 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0172-8083
No coin nor oath required. For personal study only.
✦ Synopsis
The effect of varying the chromosomal dosage in aneuploids for chromosome I or VII on the synthesis of individual nonhistone proteins was revealed. The nuclear content of most nonhistone proteins seemed to be little impaired in a strain with tetrasomy for chromosome VII as compared with a strain which is disomic for chromosome VII. On the contrary, tetrasomy for chromosome I as compared with disomy for this chromosome seems to impair both the cellular and the nuclear content of a number of chromosomal proteins, including the tubulin subunits.
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Although Saccharomyces cerevisiae strains generally have similar chromosomal band patterns as revealed by pulsed field gel electrophoresis, individual bands often move slightly differently from one strain to the other. Surveying strains from our stock collection, we found that nearly all the bands o