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Chromosomal proteins inSaccharomyces cerevisiae

✍ Scribed by Kay Gulløv; Jørgen Friis


Book ID
104777257
Publisher
Springer-Verlag
Year
1980
Tongue
English
Weight
533 KB
Volume
2
Category
Article
ISSN
0172-8083

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✦ Synopsis


Proteins were isolated from purified yeast chromatin and subjected to two-dimensional electrophoresis. The cellular and the chromosomal content of the major nonhistone proteins was measured. Two polypeptides of molecular weights 55,000 and 53,000, identified as α and β tubulin, and a polypeptide of molecular weight 63,000, associated with the nuclear DNA to a very high degree, account for nearly 50% of the nonhistone proteins present in chromatin. Only one tenth of the RNA polymerase subunit with the molecular weight of 23,000 was associated with nuclear DNA following chromatin purification in metrizamide gradients.


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