The effect of varying the chromosomal dosage in aneuploids for chromosome I or VII on the synthesis of individual nonhistone proteins was revealed. The nuclear content of most nonhistone proteins seemed to be little impaired in a strain with tetrasomy for chromosome VII as compared with a strain whi
Chromosomal proteins inSaccharomyces cerevisiae
✍ Scribed by Kay Gulløv; Jørgen Friis
- Book ID
- 104777257
- Publisher
- Springer-Verlag
- Year
- 1980
- Tongue
- English
- Weight
- 533 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0172-8083
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✦ Synopsis
Proteins were isolated from purified yeast chromatin and subjected to two-dimensional electrophoresis. The cellular and the chromosomal content of the major nonhistone proteins was measured. Two polypeptides of molecular weights 55,000 and 53,000, identified as α and β tubulin, and a polypeptide of molecular weight 63,000, associated with the nuclear DNA to a very high degree, account for nearly 50% of the nonhistone proteins present in chromatin. Only one tenth of the RNA polymerase subunit with the molecular weight of 23,000 was associated with nuclear DNA following chromatin purification in metrizamide gradients.
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