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Chromosomal location of soluble glutamic-pyruvic transaminase-1 (Gpt-1) in the mouse

✍ Scribed by Eva M. Eicher; James E. Womack

Publisher: Springer
Year: 1977
Tongue: English
Weight: 360 KB
Volume: 15
Category: Article
ISSN: 0006-2928
DOI: 10.1007/bf00484544

No coin nor oath required. For personal study only.

✦ Synopsis

Three alleles at the Gpt-1 (glutamic-pyruvic transaminase-1) locus in the mouse, as identified by electrophoresis on cellulose acetate, and their distribution among inbred mouse strains and wild stocks are described. The Gpt-1 locus was shown to control the soluble form of the enzyme. Three-point linkage analysis established the location of Gpt-1 on chromosome 15 between uw and bt. In addition, a new staining procedure is described that allows the visualization of GPT activity on gels by the deposition of formazan. This is an improvement over previous methods that produced bands of nonfluorescenee against a fuorescent background.

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